Bioorthogonal SERS Nanoprobes for Mulitplex Spectroscopic Detection,Tumor Cell Targeting,and Tissue Imaging

A surface‐enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman‐silent region of biological samples. AS1411 aptamer was also co‐conjugated with AuNF through a self‐assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small‐molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman‐silent region. These Raman nanoprobes co‐conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging.     

Synthesis and performances for treating oily wastewater produced from polymer flooding of new demulsifiers based on polyoxyalkylated N,N‐dimethylethanolamine

Usually, oily wastewater produced from polymer flooding (OWPF) was treated by cationic polymer in oilfield. In this paper, six block copolymers of ethylene oxide and propylene oxide were prepared by using N,N‐dimethylethanolamine (DMEA) as the acceptor (DMEA‐mnp and DMEA‐mnpq, n:m:p:q represented the weight ratio of different blocks). Most of DMEA products could perform well for treating OWPF, especially DMEA1231. Their performances were mainly affected by temperature. The reason of increasing oil removal performance of DMEA1231 with temperature was the decrease of interfacial dilational modulus (ε) with increasing temperature. When temperature raised up to 55°C, the ε of DMEA1231 had the minimum (1.5 l mN/m). Therefore, the OiW had the minimum (90 mg/l). Because DMEA1231 had the best performance, its flocculation kinetics was studied systemically. The results showed that the optimum condition for DMEA1231 was as follows: dosage was 300 mg/l, temperature was 55°C, stirring speed was 200 rpm and stirring time was 5 min. At last, the offshore oil field test was carried out to check the DMEA1231 performance. The result showed that at the optimum condition, DMEA1231 could perform as well as the cationic polymer. The most important thing was that flocs of DMEA1231 were not viscous and floated on the surface of the water. The results obtained by this paper provide a good choice for the treatment of OWPF in offshore oilfield to avoid the formation of viscous flocs. Copyright © 2014 John Wiley & Sons, Ltd.     

Molecular dynamics studies on the local disordering of Σ3 and Σ11 grain boundaries in aluminium bicrystal

Molecular dynamics (MD) simulations using Morse interaction potential are performed in studies of [110] symmetrical tilt grain boundary (GB) structures with mis-orientation angles 50.5°(Σ11), 129.5°(Σ11), 70.5°(Σ3) and 109.5°(Σ3) at various tempratures. The GB structures are found to start local disordering at about 0.5T _m (T _m is the melting point of aluminium) for 50.5°(Σ11), 0.32T _m for 129.5° (Σ11) and 0.38T _m for 70.5°(Σ3), respectively. These results agree with conclusions deduced from the anelastic measurements. But, for twin-boundary structure 109.5°(Σ3), this disordering has not been found even when temperature increases up to 0.9T _m . The project supported by the National Natural Science Foundation of China and Laboratory for Non-linear Mechanics of Continuous Media, Institute of Mechanics, Academia Sinica.     

Prediction of Quasi-Brittle Fracture of Concrete and Rock Structures Using Laboratory-Sized Specimens

A simple method is developed for predicting the fracture behaviour of struetures made of quasi-brittle materials sueh as eonerete and roek using the data from laboratory-sized speeimens. The method is based on the reeently-developed boundary effeet concept and associated asymptotic model. It is demonstrated that the ＂apparent＂ size dependence of fraeture behaviour of concrete and rock is in fact due to the influence of specimen boundaries. Various size effect phenomena that are often observed in fracture meehanies tests of eoncrete and roek are related to each other, and the asymptotie boundary effect model can explain all the observed ＂size＂ effeet phenomena. Four types of experimental results available in the literature （ineluding the data measured on （1） the speeimens of identical size with different crack-to-size （α） ratios, （2） specimens of different sizes with different a-ratios, （3） different types of specimens and （4） geometrieally similar speeimens） are used to verify the asymptotic boundary effect model, and it is found that the predictions of the model agree very well with the experimental results. Furthermore, the important fracture properties, fracture toughness KIC and strength f, of quasi-brittle materials sueh as eonerete and roek can also be calculated using the formulae provided in the model.     

具有多种失效模式的抗震结构的模糊可靠性分析

由于地震荷载和结构抗力具有明显的模糊性,在文献[1,2]中我们提出了具有单一失效模式的抗震结构的模糊可靠性分析.本文将上述概念和方法推广到具有多种失效模式的抗震结构,为此,又提出了结构的“模糊有效域”和“模糊失效域”的概念。     

CHARACTERIZATIONS AND RELATIONSHIPS BETWEEN OUTDOOR AND INDOOR BIOAEROSOLS IN AN OFFICE BUILDING

1.IntroductionBioaerosolsaremicroorganismsorparticles,gases,vapors,orfragmentsofbiologicalorigin(i.e.,aliveorreleasedfromalivingorganism)thatexistintheair.Bioaerosolsareeverywhereintheenvironment.Underfavorableconditions,bioaerosolsareabletogrowandpropagateonavarietyofbuildingmaterialsandindoorsurfaces,causingindoorairpollution.Therefore,owingtotheirubiquitouspresenceinnature,bioaerosolsasapollutionsourcearealmostinevitableinarangeofenclosedenvironmentsandincommunityair(Kodama&McGee,1986;Lig…     

结构拓扑优化设计的三角网格进化法

针对进化或拓扑优化方法的不足，提出了一种基于遗传算法的新型进化式拓扑优化方法－－三角网格进化法，该方法不仅能够同时进行拓扑，形状与截面变量优化设计，而且在优化过程中实现了退化和进化的统一，提高了优化效率。另外本文还首次对结构类型变量进行了优化计算，取得了有益的结果。最后几个数值算例证明了本方法的可行性和有效性。     

AuQ+碰撞氦原子单电离的全微分截面

利用三体扭曲波计算了3.6 MeV/amu的Au^Q+ (Q=24,53)碰撞氦原子单电离的全微分截面,其结果与实验数据和其它理论进行了比较,表明全微分截面受到高电荷入射粒子的强烈影响,敲出的电子被“拖拽”沿着向前的方向. 但是,所有的理论结果都不能呈现实验上全微分截面的独特的向前峰.